Improving reproductive technologies for gene banking
Upstream, the objectives of WP3 are to develop and implement methods and tools to improve the
cryoconservation and reproductive efficiency of biological samples. Downstream, its objective is to ensure the
reliability of their use in breeding, e.g. for breed reconstitution. Priority will be given to the main impediments
that hinder successful cryopreservation of genetic collections and/or hinder the use of genetic collections to
generate offspring. Opportunities to develop collections and bottlenecks highly depend on species.
WP3 will select the main technical impediments and develop innovative methods for sampling and storage.
Some methods will be adapted to different species such as gonadic storage and transfer (grafting) in birds and
mammals; or embryo cryopreservation (vitrification) and transfer for difficult mammalian resources (pigs). In
domestic birds where only semen cryopreservation is available with a too highly variable success, alternatives to
semen (PGCs, gonadic tissues in birds) will be developed and improvement in semen freezing methods will be
pursued. WP3 will then develop new approaches to better predict the reproductive success of samples stored in
WP3 will result in novel technologies to be integrated in standard-operating procedures of gene banks such as:
Methods and protocols for cryopreservation and use of semen, embryos, PGCs and gonadic tissues validated
by in-vivo reproductive measurements.
New phenomic tools for quality assessment of samples (fertility tests, biological functions, proteomic
indicators reflecting reproductive ability) validated by large scale reproductive measurements.